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KMID : 0368420050480020258
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Journal of Plant Biology
2005 Volume.48 No. 2 p.258 ~ p.262
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Genetic transformation ofAjuga multiflora bunge withAgrobacterium rhizogenes and 20-hydroxyecdysone production in hairy roots
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Kim Ok-Tae
Manickavasagm M.
Kim Young-Jun
Jin Mei Ran
Kim Kwang-Soo
Seong Nak-Sul
Hwang Baik
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Abstract
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An efficient transformation system forAjuga multiflora Bunge was established by usingAgrobacterium rhizogenes strain A4. After inoculation with the bacteria, we obtained a number of hairy-root clones from micro-calli of the explant petioles. One fast-growing line showed the highest production of 20-hydroxyecdysone (20-HE). PCR amplification of rooting locus (rol) genes revealed that the left hand-transferred DNA of the root-inducing plasmid was inserted into the genome of our transformedAjuga hairy roots. This integration was further confirmed by DNA-DNA hybridization. The 20-HE content in hairy roots was 10 times higher than that measured in the wild type.
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KEYWORD
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Ajuga multiflora, hairy roots, 20-hydroxyecdysone, Ri-plasmid, rol genes
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